Construction of Engineered Bacteria

Engineered bacteria are a type of microbial chassis that have undergone artificial rational design and genetic modification. They play an irreplaceable role in various fields such as biotechnology, industrial production, pharmaceutical R&D, and basic biological research. Common model chassis and industrial engineered bacteria mainly include Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae, Corynebacterium glutamicum, as well as a variety of non-traditional chassis microorganisms with special metabolic pathways (e.g., Pseudomonas putida, Yarrowia lipolytica).

Engineered bacteria possess highly customizable physiological and metabolic characteristics and dedicated application scenarios, demonstrating significant application advantages in different fields: As a typical model prokaryotic chassis, Escherichia coli is currently a widely used host for genetic engineering. With its clear genetic background and efficient heterologous expression capability, it occupies a core position in the large-scale production of recombinant proteins, metabolic pathway reconstruction, and synthetic biology component testing; Saccharomyces cerevisiae and non-traditional yeasts are classic eukaryotic chassis, featuring strong eukaryotic post-translational modification capabilities and flexible metabolic regulation. They can not only be used for the biosynthesis of various high-value-added natural products but also have important application potential in cell factory construction, food fermentation, and pharmaceutical intermediate production; Bacillus subtilis, as a high-quality secretory expression host, plays an indispensable role in the efficient synthesis of industrial enzymes (such as amylase), fine chemical production, and industrial biotechnology due to its strong protein secretion capacity and simple fermentation process; Engineered bacteria for live biotherapeutics (e.g., attenuated Salmonella, engineered probiotics) are important tools in the pharmaceutical field and also serve as important model systems for analyzing targeted delivery mechanisms, microecological intervention, and host-microbe interactions, providing a key platform for novel live bacterial therapies and precision medical interventions; Engineered bacteria from special habitats (e.g., stress-resistant or phage-resistant engineered bacteria) exhibit diverse application values in complex fermentation, industrial waste utilization, and agricultural biotechnology. For example, they can be used for efficient biomanufacturing and platform compound production and also have broad application prospects in the field of environmental pollutant degradation.

Service Advantages

(1) High Efficiency and Precision: Built on the CRISPR/Cas system and high-throughput DNA assembly technology, it achieves high efficiency in genome integration and regulation (the efficiency of single-gene editing or large-fragment knock-in for most chassis is significantly improved, with some approaching 100%). Precise design of regulatory elements reduces metabolic load and off-target effects.

(2) Flexibility and Diversity: It supports various construction methods, including gene knockout, multi-site knock-in, pathway reconstruction, and expression regulation (e.g., promoter replacement). It can customize expression vectors, metabolic flux guidance, and dynamic regulatory switches (e.g., light control, small-molecule induction) to adapt to the physiological characteristics of different engineered bacteria.

(3) Wide Applicability: It covers prokaryotic chassis (e.g., Escherichia coli, Bacillus species) and eukaryotic chassis (e.g., yeast-like fungi), applicable to both laboratory standard model strains and potential industrial wild strains. It supports marker-free genome editing and the screening of stably heritable clones.

(4) Controllable Cycle: The project cycle for constructing standard chassis strains is usually 1-4 months (1-2 months for conventional model strains, and 2-4 months for complex metabolic pathway reconstruction or non-traditional chassis), with standardized processes ensuring reliable results.

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