GeneRulor pG-Transposase

1. Product Overview

GeneRulor pG-Transposome is a transposome prepared from pG-Transposase. In comparison with GeneRulor pA-Transposome, the design of GeneRulor pG-Transposome further expands the antibody binding range. Its Protein G domain not only has broad affinity for antibodies from multiple species including rabbit, mouse and goat, but also shows a significant advantage in binding certain key subtypes, especially mouse IgG1, thus achieving strong and stable binding to a wider range of antibody types. This characteristic makes GeneRulor pG-Transposome have higher versatility and experimental adaptability in targeted chromatin analysis technologies such as CUT&Tag and CUT&RUN.

This tool completely retains the inherent DNA cleavage and ligation functions of transposase, and at the same time, with the enhanced antibody binding capacity of Protein G, it can flexibly adapt to antibodies of different host origins and subtypes. Therefore, GeneRulor pG-Transposome provides a more comprehensive and reliable solution for the research on epigenetics, chromatin interactions and transcriptional regulation, and is particularly suitable for experimental systems using mouse IgG1 antibodies or requiring cross-species compatibility.

2. Product Features

(1) Broad-spectrum antibody affinity: Fused with protein G, it has high affinity for multiple IgG antibody subtypes;

(2) Enhanced targeting ability: It has higher antibody binding stability and specificity compared with pA-Transposase;(3) Ultra-low sample requirement: It can effectively process samples with as few as 500 cells;

(4) Ultra-high sensitivity: It has sub-nanometer resolution for chromatin modifications and transcription factor binding sites;

Low background noise: Optimized enzymatic properties significantly reduce non-specific cleavage and improve the signal-to-noise ratio.

3. Applications

Epigenetics Research

Enhanced CUT&Tag technology: Through its excellent antibody affinity, pG-Transposase achieves ultra-precise labeling of regions of interest and efficient library construction. Its working principle is to utilize the strong interaction between protein G and the Fc segment of IgG antibody to guide transposase to target sites more firmly, realizing highly specific DNA fragmentation and adaptor ligation. This technology is particularly suitable for studying extremely low-abundance transcription factor binding sites and rare histone modifications, and can obtain high-quality data with lower cell input, enabling single-cell level epigenomic analysis.

Figure 1. Flow chart of CUT&Tag experiment using pG-Transposase

Next-Generation Sequencing (NGS) Library Construction

GeneRulor Transposase is a highly efficient tool for constructing DNA libraries for next-generation sequencing. It is characterized by utilizing the special transposition mechanism of GeneRulor Transposase dimer to simultaneously complete two steps: double-stranded DNA cleavage and adaptor ligation. This enzyme has rapid reaction capacity and requires a small amount of samples, and can fragment DNA and add specific adaptor sequences at both ends in a single reaction. This simplified "one-step" tagging method greatly optimizes the experimental process, which not only saves operation time but also reduces experimental costs, thus being widely used in the field of DNA sequencing library construction.

Figure 2. Schematic diagram of next-generation sequencing library construction

References

[1] Li N, Jin K, Bai Y, Fu H, Liu L, Liu B. Tn5 Transposase Applied in Genomics Research. Int J Mol Sci. 2020 Nov 6;21(21):8329. doi: 10.3390/ijms21218329.

[2] Zhang H, Lu T, Liu S, Yang J, Sun G, Cheng T, Xu J, Chen F, Yen K. Comprehensive understanding of Tn5 insertion preference improves transcription regulatory element identification. NAR Genom Bioinform. 2021 Oct 27;3(4):lqab094. doi: 10.1093/nargab/lqab094.