Mouse Cot‑1 DNA is genomic DNA extracted from mouse liver, with fragment sizes mainly ranging from 50 to 300 bp. It is rich in rodent‑specific repetitive DNA sequences such as B1, B2, and L1 families, and serves as a core blocking reagent for rodent‑related nucleic acid hybridization experiments. It can effectively block non‑specific hybridization in microarray screening. Moreover, it suppresses non‑specific binding of rodent repetitive DNA sequences during In situ hybridization and Southern blot mapping of rodent clones, and accurately identifies mouse clones in somatic cell hybrid libraries derived from mouse‑hamster hybrid cells. This product adopts a unique fragmentation process, ensuring that more than 50% of fragments fall within the optimal range of 50–300 bp, with a blocking efficiency of over 95%. It also features strict quality control standards and high batch‑to‑batch consistency, which significantly reduce experimental background signals and improve result accuracy. It provides reliable support for various rodent‑related experiments, including Northern hybridization and liquid-phase hybridization capture.
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Mouse Cot-1 DNA
1. Product Introduction
Mouse Cot‑1DNA is extracted from mouse liver DNA, with most fragments ranging from 50 to 300 bp. It is rich in repetitive DNA sequences, such as B1, B2, and L1 family members. Mouse Cot‑1 DNA is commonly used to block non‑specific hybridization in microarray screening. It can also be used to suppress hybridization of rodent repetitive DNA sequences (when mapping rodent clones by in situ hybridization or Southern blotting) and to identify mouse clonesin somatic cell hybrid library screening derived from mouse‑hamster hybrid cells.
2. Applications
Mouse Cot-1 DNA is mainly used in Northern blotting, Southern blotting, in situ hybridization (tissue In situ hybridization, somatic cell In situ hybridization, fluorescence In situ hybridization), and liquid-phase hybridization capture. In addition, Mouse Cot-1 DNA can also be used as a library screening probe for the screening of somatic cell hybrid libraries and flow-sorted chromosome libraries constructed from somatic cell hybrids.
Figure 1. Application scenarios of Mouse Cot‑1 DNA
References
[1] Bao P, Frutos A. G, Greef C .et al.Anal Chem (2002) 74:1792-1799.
[2] Tang Y M, Wo Y Y,Stewart J,.et al.JBiol Chem (1996) 271:28324-28354.
[3] Trifonov V.A, Vorobieva N.N, RensW. Springer (2009): FISH With and Without COT1 DNA.
